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KMID : 0921620230530010043
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Journal of Bacteriology and Virology
2023 Volume.53 No. 1 p.43 ~ p.50
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Isolation and Biomolecular Analysis of Canine Parvovirus 2a from Korean Dogs
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Cheng-Hsun Lu
Yang Dong-Kun
Kim Eun-Ju
Park Yu-Ri
Lee Hye-Jeong
Hyun Bang-Hun
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Abstract
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Canine parvovirus (CPV) is highly contagious and causes acute hemorrhagic gastroenteritis in puppies. Four variants of CPV-2 are widely distributed worldwide: CPV-2, CPV-2a, CPV-2b, and CPV-2c. However, there have been few molecular characterization studies of CPV-2a circulating in dog populations in South Korea. Here, we isolated and characterized two CPVs: the CPV2101 and CPV2102 isolates. They were confirmed to be CPVs based on their cytopathic effects, indirect fluorescence assays, electron microscopy, real-time PCR, and sequence analysis. Both isolates showed hemagglutination activity with pig erythrocytes and had viral titers ranging from 106.5 to 105.5 FAID50/mL in A72 cells. The nucleotide and amino acid sequences of VP2 of the isolates were compared with reported CPV strains. The sequence alignment showed that they had 100% nucleotide sequence similarity with each other and grouped with CPV-2a. They had mutations at positions 267, 322, 324, and 440 in the VP2 gene compared to the original CPV-2a strains. A phylogenetic analysis of the VP2 gene of the isolates revealed that they were closely related to the K01708-01 strain reported from South Korea. The two isolates will serve as useful antigens for developing new CPV vaccines.
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KEYWORD
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Canine parvovirus, Dogs, VP2, Phylogenetic analysis
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